Featured Publications from UW Bioengineering
Researchers from Shaoyi Jiang's (BioE adjunct) lab demonstrate a zwitterionic polycarboxybetaine nanocage that can physically encase proteins while keeping their structure intact. This approach addresses the problem posed by efficacy loss of biotherapeutics due to undesirable immune response.
The Gao lab reports on the development of small, bifunctional chemical tags capable of transporting siRNA directly into the cytosol. The bifunctional tags consist of a siRNA-binding moiety that interacts with siRNA non-covalently, and a steroid domain that readily fuses with the mammalian cell membrane.
Deok-Ho Kim's lab demonstrates that biomimetic nanotopography and S1P can be combined to synergistically regulate the maturation and vascularization of engineered skeletal muscles.
Increased Calcific Aortic Valve Disease in response to a diabetogenic, procalcific diet in the LDLr-/-ApoB100/100 mouse model
The Scatena and Giachelli labs developed an animal model that mimicked the structural and functional features of CAVD in people with T2DM, by testing a diabetogenic, procalcific diet and its effect on the incidence and severity of CAVD and AS in the, LDLr-/-ApoB100/100 mouse model.
Comparison of Neovascular Lesion Area Measurements From Different Swept-Source OCT Angiographic Scan Patterns in Age-Related Macular Degeneration
The researchers compared area measurements for the same neovascular lesions imaged using swept source optical coherence tomography angiography (SS-OCTA) and enlarging scan patterns. The similarity in lesion area measurements across different scan patterns suggests that SS-OCTA imaging can be used to follow quantitatively the enlargement of choroidal neovascularization as the disease progresses.
Current approaches for topical vaginal administration of nanoparticles result in poor retention and extensive leakage. To overcome these challenges, the researchers developed a nanoparticle-releasing nanofiber delivery platform and evaluated its ability to improve nanoparticle retention in a murine model. In this first study of nanoparticle releasing fibers for drug delivery, they discovered that the system demonstrated 30-fold increased nanoparticle retention in the reproductive tract 24 hours following administration.
A new contrast agent for combined photoacoustic and ultrasound imaging is presented.
The researchers achieved high-throughput, quantitative characterization of protein–protein binding interactions without requiring purified recombinant proteins, by linking interaction strength with yeast mating. Using a next-generation sequencing output, they characterized protein networks consisting of thousands of pairwise interactions in a single tube and have demonstrated the effect of changing the binding environment.
Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue
The researchers demonstrate the application of crosslinking mass spectrometry to identify protein structural features and interactions in tissue samples, providing systems structural biology insight into protein complexes as they exist in the mouse heart. The extension of crosslinking mass spectrometry analysis into the realm of tissues opens the door to increasing understanding of protein structures and interactions within the context of the greater biological system.
Loss of PiT-2 results in abnormal bone development and decreased bone mineral density and length in mice
The study's findings suggest that PiT-2 is involved in normal bone development and growth and plays roles in cortical and trabecular bone metabolism feasibly by regulating local phosphate transport and mineralization processes in the bone. Further studies that evaluate bone cell-specific loss of PiT-2 are now warranted and may yield insight into complex mechanisms of bone development and growth, leading to identification of new therapeutic options for patients with bone diseases.