Cas9 as a Versatile Tool for Engineering Biology
Dr. Prashant Mali
Harvard University Medical School
January 28, 2014
Foege N130A, Wallace H. Coulter Seminar Room
RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular organization, regulation, and behavior. Here I will describe my work on developing the Cas9 targeting methodology, and detail current and prospective engineering advances with a focus on developing systems approaches for regenerative medicine applications.
Dr. Prashant Mali received a Bachelors and Masters in Electrical Engineering from the Indian Institute of Technology (Bombay), with a research focus on biomedical devices and micro-fabrication; and a doctorate in Biomedical Engineering at the Johns Hopkins University, with a research focus on pluripotent stem cell biology. Presently Dr. Mali is pursuing a postdoctoral fellowship at Harvard Medical School, with projects focusing on mammalian genome & cell engineering.