Cationic polymers for non-viral gene delivery to human T cells
Suzie Pun and colleagues We demonstrate transfection of both CD4+ and CD8+ primary human T cells with messenger RNA and plasmid DNA at efficiencies up to 25 and 18%, respectively, with similarly high viability.
A Carboxylate to Amide Substitution That Switches Protein Folds
Valerie Daggett and colleagues performed a comparative molecular dynamics study on the denatured states of two proteins, sharing nearly identical amino?acid sequences (88?%) but different topologies, namely an all?a?helical bundle protein named GA88 and an a+B?protein named GB88.
Analysis of the Myc-induced pancreatic B cell islet tumor microenvironment using imaging ToF-SIMS.
Lara Gamble and colleagues demonstrate that ToF-SIMS can be utilized to identify large-scale changes that occur in the tumor microenvironment and could thereby increase the understanding of tumor progression and the tumor microenvironment.
Macrophage-targeted drugamers with enzyme-cleavable linkers deliver high intracellular drug dosing and sustained drug pharmacokinetics against alveolar pulmonary infections
Patrick Stayton and colleagues demonstrate the versatility of the drugamer platform for engineering the intracellular pharmacokinetic profiles and its strong therapeutic activity in treating pulmonary intracellular infections.
A ribonucleoprotein octamer for targeted siRNA delivery
Professor Xiaohu Gao and colleagues have created a new way to target prostate tumors that overcomes past challenges of designing effective drug delivery methods. This versatile nanocarrier design should offer opportunities for the clinical translation of therapies based on intracellularly acting biologics.
Mobile Phone Ratiometric Imaging Enables Highly Sensitive Fluorescence Lateral Flow Immunoassays without External Optical Filters
Professor Paul Yager's lab has created a method that enables optical-filter free mobile imaging for medical diagnostics, a first step towards enabling a new generation of highly sensitive, point-of-care fluorescence assays.