Emeritus Professor
castner@uw.edu
Phone: (206)543-8094
Office: MolES 225
UW Bioengineering faculty David Casnter

David G. Castner

Lab Website

How I am inventing the future of medicine

The objective of my research is to improve our understanding of the relationship between the surface properties of a biomaterial and the biological reactions occurring on that material.

Biomedical Surface Analysis
Surface & Interface Characterization
Surface Modification

Our research is focused on determining in detail the surface structure and composition of a material, as well as the interaction of biomolecules with the surface of materials. Surface analysis methods used include x-ray photoelectron spectroscopy, static time-of-flight secondary ion mass spectrometry, scanning force microscopy, near edge x-ray adsorption spectroscopy, sum frequency generation and surface plasmon resonance. Current research projects include the characterization of model biomaterial systems (self-assembled monolayers, peptides, ordered proteins, biosensors, DNA microarrays, etc.), the development of 2D and 3D chemical state imaging methods for analysis of organic and biological samples, and characterization of surface immobilized biomolecules.

PhD, Physical Chemistry, University of California at Berkeley, 1979
BS, Chemistry, Oregon State University, 1975

2018, Medard W. Welch Award, AVS
2017, ECASIA Award
2014, Rivière Prize, UK Surface Analysis Forum
2013 Fellow, American Institute of Medical and Biological Engineering
2003-2017 Visiting Professor, Université Paris 13
2009-2011 President-Elect (09), President (10), Immediate Past President (11), American Vacuum Society
2005-2007 Board of Directors, American Vacuum Society
2004 Program Chair, American Vacuum Society International Symposium
2004 Clemson Award for Basic Research, Society for Biomaterials
2004 Fellow, Biomaterials Science and Engineering
2003 Excellence in Surface Science Award, Surfaces in Biomaterials Foundation
1999 Fellow, American Vacuum Society

BIOEN 493/593: Advanced Surface Analysis

“Biomedical Surface Analysis:  Evolution and Future Directions,” D.G. Castner, Biointerphases, 12, paper 02C301 (11 pages), 2017.

“Predicting the Orientation of Protein G B1 on Hydrophobic Surfaces using Monte Carlo Simulations,” E.T. Harrison, T. Weidner, D.G. Castner and G. Interlandi, Biointerphases, 12, paper 2D401 (10 pages), 2017.

“Multi-technique Characterization of Oligo(ethylene glycol) Functionalized Gold Nanoparticles,” A. Rafati, A.G. Shard and D.G. Castner, Biointerphases, 11, paper 04B304 (10 pages), 2016.

“ToF-SIMS and XPS Characterization of Protein Films Adsorbed onto Bare and Sodium Styrene Sulfonate Grafted Gold Substrates,” R.N. Foster, E.T. Harrison and D.G. Castner, Langmuir, 32, 3207-3216, 2016.

“Quantifying the Impact of Nanoparticle Coatings and Non-uniformities on XPS Analysis:  Gold/silver Core-shell Nanoparticles,” Y.-C. Wang, M.H. Engelhard, D.R. Baer and D.G. Castner, Analytical Chemistry, 88, 3917-3925, 2016.

“Differential Surface Activation of the A1 Domain of von Willebrand Factor,” E.H. Tronic, O. Yakovenko, T. Weidner, J.E. Baio, R. Penkala, D.G. Castner and W.E. Thomas, Biointerphases, 11, paper 029803 (9 pages), 2016.

“A Technique for Calculation of Shell Thicknesses for Core-Shell-Shell Nanoparticles from XPS Data,” D.J.H. Cant, Y.-C. Wang, D.G. Castner and A.G. Shard, Surface and Interface Analysis, 48, 274-282, 2016.

“Three-dimensional Localization of Polymer Nanoparticles in Cells using ToF-SIMS,” D.J. Graham, J.T. Wilson, J.J. Lai, P.S. Stayton and D.G. Castner, Biointerphases, 11, paper 02A304 (6 pages), 2016.

“ToF-SIMS 3D Imaging of Native and Non-Native Species within HeLa Cells,” J. Brison, M.A. Robinson, D.S.W. Benoit, S. Muramoto, P.S. Stayton and D.G. Castner, Analytical Chemistry, 85, 10869-10877, 2013.

“SFG analysis of surface bound proteins: A route towards structure determination,” T. Weidner and D.G. Castner, Physical Chemistry Chemical Physics, in press, 2013.

“Multivariate Analysis of ToF-SIMS Data from Multicomponent Systems: The Why, When, and How,” D.J. Graham and D.G. Castner, Biointerphases, 7, 2012, paper 49.

“NEXAFS Analysis of Individual Phenylalanine Orientations in Statherin Bound to Hydroxyapatite,” T. Weidner, M. Dubey, N.F. Breen, J. Ash, J. E. Baio, C. Jaye, D.A. Fischer, G.P. Drobny and D.G. Castner, J. American Chemical Society, 134, 8750-8753, 2012.

“ToF-SIMS Depth Profiling of Cells: Z-correction, 3D Imaging, and Sputter Rate of Individual NIH/3T3 Fibroblasts,” M.A. Robinson, D.J. Graham and D.G. Castner, Analytical Chemistry, 84, 4880-4885, 2012.

“Probing the Orientation of Electrostatically Immobilized Protein G B1 by Time of Flight Secondary Ion Spectrometry, Sum Frequency Generation and Near-edge X-Ray Adsorption Fine Structure Spectroscopy,” J.E. Baio, T. Weidner, L. Baugh, L.J. Gamble, P.S. Stayton and D.G. Castner, Langmuir, 28, 2107-2112, 2012.

“Exploring the Surface Sensitivity of ToF-SIMS by Measuring the Implantation and Sampling Depths of Bin and C60 Ions in Organic Films,” S. Muramoto, J. Brison and D.G. Castner, Analytical Chemistry, 84, 365-372, 2012.

“ToF-SIMS Analysis of Adsorbed Proteins: Principal Component Analysis of the Primary Ion Species Effect on the Protein Fragmentation Patterns” S. Muramoto, D.J. Graham, M.S. Wagner, T.G. Lee, D.W. Moon and D.G. Castner, Journal of Physical Chemistry C, 115, 24247-24255, 2011.

“Simulation and Modeling of Self-Assembled Monolayers of Carboxylic Acid Thiols on Flat and Nanoparticle Gold Surfaces,” S. Techane, D.R. Baer and D.G. Castner, Analytical Chemistry, 83, 6704-6712, 2011.

“Multi-technique Characterization of Self-assembled Carboxylic Acid Terminated Alkanethiol Monolayers on Nanoparticle and Flat Gold Surfaces,” S.D. Techane, L.J. Gamble and D.G. Castner, Journal of Physical Chemistry C, 115, 9432-9441, 2011.

“A Sum Frequency Generation and Solid-State NMR Study of the Structure, Orientation and Dynamics of Polystyrene-Adsorbed Peptides,” T. Weidner, N.F. Breen, K. Li, G.P. Drobny and D.G. Castner, Proceedings of the National Academy of Sciences, 107, 13288-13293, 2010.

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