Office: Foege N210E
Hao Yuan Kueh
We lay foundations for engineering immune cells to fight cancer and other life-threatening diseases.
Systems, synthetic and quantitative biology
Molecular and cellular engineering
Stem cell and developmental biology
Cell cycle regulation
Our lab studies how immune cells make fate decisions, both as they develop from stem cells, and as they respond to antigens. To understand the molecular circuitry controlling immune cell fate choice, we follow its dynamic behavior at the single-cell level using live imaging, uncover its molecular components using modern genetic, biochemical and high-throughput approaches, and elucidate underlying design principles using mathematical modeling. We then use this understanding to engineer molecular circuits for precise control of immune cell function. Through this work, we aim to lay foundations for programming immune cells to fight cancer and other life-threatening diseases.
Ph.D., Biophysics, Harvard University
A.B., Physics, Princeton University
Postdoctoral Scholar, California Institute of Technology
2014- K99/R00 Pathway to Independence Award, NIH
2011 Postdoctoral fellowship, Irvington / Cancer Research Institute
2006 Award for Distinction in Teaching, Harvard University
2002 Predoctoral fellowship, Howard Hughes Medical Institute
Kueh H.Y., Yui M.A., Ng K., Pease S., Siu S., Bernstein I.D., Elowitz M.B., Rothenberg E.V. (2016). Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment. Nature Immunology, 17:956-65.
Rothenberg E.V., Kueh H.Y., Yui M.A., Zhang J. (2016). Hematopoiesis and T-cell specification as a model developmental system. Immunol Rev. 271(1)72-97.
Kueh H.Y., Champhekar A., Nutt S.L., Elowitz M.B., Rothenberg E.V. (2013) Positive feedback between PU.1 and the cell cycle controls myeloid differentiation. Science, 341(6146):670-3
Kueh H.Y., Rothenberg E.V. (2012) Regulatory gene network circuits underlying T-cell development from multipotent progenitors. Wiley Interdiscip Rev Syst Biol Med, 4(1):79-102
Kueh H.Y., Brieher W.M., Mitchison T.J. (2010) Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover. Biophys J, 99(7): 2153-62
Kueh H.Y., Mitchison T.J., Brieher W.M. (2008) Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. Journal of Cell Biology, 182(2):341.
Levine J., Kueh H.Y., Mirny L (2007). Intrinsic fluctuations, robustness, and tunability in signaling cycles. Biophysical Journal, 92(12):4473-81.
Vilar J.M., Kueh H.Y., Barkai N., Leibler S. (2002). Mechanisms of noise-resistance in genetic oscillators. Proceedings of the National Academy of Sciences, 99(9):5988-92.