Project Description

Phone: (206)221-5876
Office: Foege N310C

UW Bioengineering faculty James Bryers

We are developing biomolecular and biomaterial technologies to prevent infections, engineer immunity, and manipulate the healing process.
Engineering infection immunity
Nucleic acid vaccine delivery
Anti-biofilm based biomaterials technologies
Anti-Biofilm Biomaterials
This NIH/NIBIB supported research seeks to develop polymer biomaterials that prevent bacterial colonization and subsequent biofilm formation by using novel small molecules that do not kill bacteria but rather negate biofilm formation. Biomaterials will release a variety of novel “anti-biofilm” therapies rather than employing a toxic compound such as an anti-biotic or disinfectant. Such therapies include: gallium-siderophore complexes that interfere with iron metabolism, DNAse enzymes that disrupt the biofilm exopolymer matrix, specific species adhesion blockers.

Engineering Infection Immunity
This group of NIH funded research projects seeks to generate biomaterials that self-vaccinate the host in order to prevent medical device based infections. Biomaterial scaffolds will be created that can control dendritic cell (DCs) activation and efficiently transfect DCs with either pDNA or mRNA nucleic acid vaccines.

Healing by Controlling Macrophage Plasticity
Can certain macrophage phenotypes continue to differentiate in desired tissue cells, exhibiting stem-cell like behavior? If so, can biomaterials be designed to engineer macrophage phenotype and affect such plasticity?

PhD, Chemical Engineering, Rice University, 1980
BS, Chemical Engineering, University of Houston, 1969
2004 College of Fellows, American Institute of Medical and Biological Engineering, Washington, DC.
1996 Hunter Distinguished Visiting Professor, Department of Bioengineering, Clemson University
1996 Speaker, Biofilm Infections, Gordon Conference on Biomaterials
1994-present Member Board of Review Editors, Biotechnology & Bioengineering, J. Wiley Publ
1997 Session Chair, Infection and Inflammation, Gordon Conference on Biomaterials
1992-1997 J. Monod Visiting Professorship, Institute Pasteur, Paris France

BIOEN 335: Mass Transport and Kinetics in Biological Systems (BioTransport II)
BIOEN 490: Engineering Materials for Biomedical Applications
BIOEN 498/599: Biofilm Engineering
Katzenmeyer KN, Bryers JD. Multivalent artificial opsonin for the recognition and phagocytosis of Gram-positive bacteria by human phagocytes, Biomaterials. 32(16): 4042-4051. (2011). PMID: 21388677.

Garty S, Shirakawa R, Warsen A, Anderson EM, Noble ML, Bryers JD, Ratner BD, Shen. TT. Sustained antibiotic release from an intraocular lens-hydrogel assembly for cataract surgery, Invest Ophthalmol Vis Sci. 52(9):6109-6116 (2011). PMID: 21447687.

Valdes, TI, Ciridon, W, Ratner, BD, Bryers, J.D. Modulation of fibroblast inflammatory response by surface modification of a perfluorinated ionomer, Biointerphases 6(2):43-53, (2011). PMID: 21721839.

Manganiello, MJ, Cheng, C, Convertine, AJ, Bryers, JD, Stayton, PS. Diblock copolymers with tunable pH transitions for gene delivery, Biomaterials 33: 2301-2309 (2012). PMID: 22169826.

Ruckh TT, Oldinski RA, Carroll DA, Mikhova K, Bryers JD, Popat KC. Antimicrobial effects of nanofiber poly(caprolactone) tissue scaffolds releasing rifampicin. J Mater Sci Mater Med., 23(6):1411-20. (2012). PMID: 22407002.

Park KR, Bryers JD. Effect of macrophage classical (M1) activation on implant-adherent macrophage interactions with Staphylococcus epidermidis: A murine in vitro model system. J Biomed Mater Res A. (2012) 100(8):2045-2053. PMID: 22581669.

Linnes JC, Mikhova K, Bryers JD. Adhesion of Staphylococcus epidermidis to biomaterials is inhibited by fibronectin and albumin. J Biomed Mater Res A. 100(8):1990-1997 (2012) PMID: 22566405.

Bryers JD, Giachelli CM, Ratner BD. Engineering biomaterials to integrate and heal: The biocompatibility paradigm shifts. Biotechnol Bioeng. 2012 109(8):1898-1911. PMID: 22592568.

Ma H, Bryers JD. Non-invasive determination of conjugative transfer of plasmids bearing antibiotic-resistance genes in biofilm-bound bacteria: effects of substrate loading and antibiotic selection. Appl Microbiol Biotechnol. (2012) Jun 6. Epub ahead of print PMID: 22669634.

Cheng C, Convertine AJ, Stayton PS, Bryers JD. Multifunctional triblock copolymers for intracellular messenger RNA delivery. Biomaterials. (2012) 33(28): 6868–6876. PMID: 22784603.

Cooper A, Oldinski R, Ma H, Bryers JD, Zhang M. Chitosan-based nanofibrous membranes for antibacterial filter applications. Carbohydr Polym. 2013. 92(1):254-259. PMID: 23218292.

Galperin A, Oldinski RA, Florczyk SJ, Bryers JD, Zhang M, Ratner BD. Integrated Bi-Layered Scaffold for Osteochondral Tissue Engineering. Adv Healthc Mater. 2012. PMID: 23225568.

Park J, Wu CT, Bryers JD. Chemokine programming dendritic cell antigen response: part I – select chemokine programming of antigen uptake even after maturation. Immunology. 2013. 139(1):72-87. PMID: 23278719.

Park J, Bryers JD. Chemokine Programming Dendritic Cell Antigen Response: Part II – Programming Antigen Presentation to T Lymphocytes by Partially Maintaining Immature Dendritic Cell Phenotype. Immunology. 2013. 139(1):88-99. PMID: 23277917.

Linnes JC, Ma H, Bryers JD. Giant Extracellular Matrix Binding Protein Expression in Staphylococcus epidermidis is Regulated by Biofilm Formation and Osmotic Pressure. Curr Microbiol. 2013 Jun;66(6):627-633. PMID: 23380801.

Ma H, Katzenmeyer KN, Bryers JD. Non-invasive in situ monitoring and quantification of TOL plasmid segregational loss within Pseudomonas putida biofilms. Biotechnol Bioeng. 2013 May 1. [Epub ahead of print] PMID: 23633286.

Ma H, Darmawan ET, Zhang M, Zhang L, Bryers JD. Development of a poly (ether urethane) system for the controlled release of two novel anti-biofilm agents based on gallium or zinc and its efficacy to prevent bacterial biofilm formation. Journal of Controlled Release 2013. 172:1035–1044. PMID: 24140747.

Yan L, Zhang L, Ma H, Chiu D, Bryers JD. A Single B-repeat of Staphylococcus epidermidis Accumulation-Associated Protein Induces Protective Immune Responses In An Experimental Biomaterial-Associated Infection Mouse Model. Clin Vaccine Immunol. 2014 Jun 11. EarlyView. PMID: 24920603.

Hopping G, Kellock J, Barnwal RP, Law P, Bryers JD, Varani G, Caughey B, Daggett V. Designed ?-sheet peptides inhibit amyloid formation by targeting toxic oligomers. eLife 2014;3:e01681. .


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